Mexico name injured Raul Jimenez in World Cup squad
Jimenez has only played four matches this season for Premier League Wolverhampton Wanderers after sustaining knee and groin injuries.
Martino left Mexico's all-time leading scorer Javier Hernandez (34) out of the final squad.
Real Betis midfielder Andres Guardado (36) will lead Mexico in his fifth World Cup after becoming his country's most capped international.
Mexico will be without Sevilla forward Jesus Corona (29) due to an ankle injury and youngsters Santiago Gimenez (21) and Diego Lainez (22) did not make the cut.
Mexico play Sweden in their last warm-up match on Wednesday before heading to Qatar where they are in Group C along with Poland, Argentina and Saudi Arabia.
Mexico Squad
Goalkeepers
Guillermo Ochoa (America)
Alfredo Talavera (Juarez)
Rodolfo Cota (Leon)
Defenders
Jorge Sanchez (Ajax)
Kevin Alvarez (Pachuca)
Nestor Araujo (America)
Johan Vasquez (Cremonese)
Gerardo Arteaga (KRC Genk)
Hector Moreno (Monterrey)
Cesar Montes (Monterrey)
Jesus Gallardo (Monterrey)
Midfielders
Erick Gutierrez (PSV)
Orbelin Pineda (AEK Athens)
Hector Herrera (Houston Dynamo)
Andres Guardado (Real Betis)
Roberto Alvarado (Guadalajara)
Edson Alvarez (Ajax)
Luis Chavez (Pachuca)
Luis Romo (Monterrey)
Carlos Rodriguez (Cruz Azul)
Uriel Antuna (Cruz Azul)
Forwards
Hirving Lozano (Napoli)
Rogelio Funes Mori (Monterrey)
Henry Martin (America)
Raul Jimenez (Wolverhampton Wanderers)
Alexis Vega (Guadalajara)